Difference between revisions of "Part:BBa K3515003"

Latest revision as of 14:26, 25 May 2020

Troponin C, a calcium binding protein with cysteine modification(s) to bind to a biosensor.

Troponin C selectively binds calcium and magnesium in its active site, inducing a conformation change in the N and C termini regions, respectively. This makes it a distinguishable candidate for in vivo or in vitro calcium monitoring using fluorescence resonance energy transfer (FRET). Coupling this protein with two fluorophores can permit calcium detection. Special considerations must be made to the active sites of Troponin C to selectively bind calcium in its active sites and avoid magnesium binding. Calcium detection is vital as calcium is used in cells to play an important role in signal transduction pathways. Calcium is often a secondary messenger and also functions as a key neurotransmitter. Calcium is also a crucial biomarker used in clinical medicine for tracking the progression and status of patients with hypocalcemia which can lead to osteoporosis or hyperparathyroidism. As such a biosensor for calcium ion tracking may be of great interest to patients and clinicians. This part includes a mutated Troponin C to have a cysteine modification that will bind cysteine linker arms and be used for biosensor immobilization allowing the detection of calcium.

Cysteine immobilization modifications of binding protein. Protein structures were obtained from the RCSB Protein Data Bank. All protein residues are shown in a blue cartoon preset with cysteine residues labelled in red cartoon, respectively, using PyMOL software.

Sequence and Features