Difference between revisions of "Part:BBa K3515005"
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Glucose/Galactose binding protein (GGBP) selectively binds glucose in its active site, inducing a conformation change in the N and C termini regions, respectively. This makes it a distinguishable candidate for in vivo or in vitro glucose monitoring using fluorescence resonance energy transfer (FRET). Coupling this protein with two fluorophores can permit glucose detection. Glucose detection is vital as glucose is used in cells to play an important role in critical metabolic pathways, which is crucial for maintaining energy homeostasis. Glucose is also a crucial biomarker used in clinical medicine for tracking the progression and status of patients with diabetes mellitus which may provide insights into pancreatic functioning and metabolic state. As such a biosensor for glucose tracking may be of great interest to patients and clinicians. This part includes a mutated GGBP to have a cysteine modification that will bind cysteine linker arms and be used for biosensor immobilization allowing the detection of glucose. | Glucose/Galactose binding protein (GGBP) selectively binds glucose in its active site, inducing a conformation change in the N and C termini regions, respectively. This makes it a distinguishable candidate for in vivo or in vitro glucose monitoring using fluorescence resonance energy transfer (FRET). Coupling this protein with two fluorophores can permit glucose detection. Glucose detection is vital as glucose is used in cells to play an important role in critical metabolic pathways, which is crucial for maintaining energy homeostasis. Glucose is also a crucial biomarker used in clinical medicine for tracking the progression and status of patients with diabetes mellitus which may provide insights into pancreatic functioning and metabolic state. As such a biosensor for glucose tracking may be of great interest to patients and clinicians. This part includes a mutated GGBP to have a cysteine modification that will bind cysteine linker arms and be used for biosensor immobilization allowing the detection of glucose. | ||
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Revision as of 18:25, 22 May 2020
Glucose binding protein with cysteine modification(s) to bind to a biosensor.
Glucose/Galactose binding protein (GGBP) selectively binds glucose in its active site, inducing a conformation change in the N and C termini regions, respectively. This makes it a distinguishable candidate for in vivo or in vitro glucose monitoring using fluorescence resonance energy transfer (FRET). Coupling this protein with two fluorophores can permit glucose detection. Glucose detection is vital as glucose is used in cells to play an important role in critical metabolic pathways, which is crucial for maintaining energy homeostasis. Glucose is also a crucial biomarker used in clinical medicine for tracking the progression and status of patients with diabetes mellitus which may provide insights into pancreatic functioning and metabolic state. As such a biosensor for glucose tracking may be of great interest to patients and clinicians. This part includes a mutated GGBP to have a cysteine modification that will bind cysteine linker arms and be used for biosensor immobilization allowing the detection of glucose.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]