Difference between revisions of "Part:BBa K3515004:Design"
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===References=== | ===References=== | ||
| + | Shen, Y., Wu, S.Y., Rancic, V., Aggarwal, A., Qian, Y., Miyashita, S.I., Ballanyi, K., Campbell, R.E. and Dong, M., 2019. Genetically encoded fluorescent indicators for imaging intracellular potassium ion concentration. Communications biology, 2(1), pp.1-10. | ||
Latest revision as of 18:20, 22 May 2020
Potassium binding protein with cysteine modification(s) to bind to a biosensor.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 55
Illegal AgeI site found at 112
Illegal AgeI site found at 172 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Added a cysteine for immobilization (A26C) opposite to the active site region for linker arm immobilization. Amino acid substitution considerations were made using an intensive BLAST search to ensure conservation.
Source
The source of this part is its sequence retrieved from the European Nucleotide Archive (U00096.3) along with our own modifications.
References
Shen, Y., Wu, S.Y., Rancic, V., Aggarwal, A., Qian, Y., Miyashita, S.I., Ballanyi, K., Campbell, R.E. and Dong, M., 2019. Genetically encoded fluorescent indicators for imaging intracellular potassium ion concentration. Communications biology, 2(1), pp.1-10.