Difference between revisions of "Part:BBa J100534:Experience"

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Data was derived by dividing fluorescence of each sample by optical density once readings had been collected from the spectrophotometer. The collected data showed a general trend of RFP production being hindered by incubating each experimental group in 42 degrees Celsius for one hour. The control experimental groups produced slightly more RFP. This suggests that the heat may have denatured the promoter and reduced its functioning capacity. The original X colonies were derived from red cells produced after we prepared our bacterial cells. Three red cells were produced in the experimental colony, which we used for the X1, X2, and X3 samples. The X1 colony was grown from a cell that was very faintly red; this could account for the fact that little RFP was produced in the presence and absence of heat. Therefore, we decided to exclude the X1 data from the X control and X heat averages on the second graph pictured above.