Difference between revisions of "Part:BBa K3320007"
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The promoter J23111 constitutively produces the alpha-hemolysin secretion system from Escherichia coli. The system is composed of HlyB, an internal membrane protein, HlyD, a membrane fusion protein, and TolC, an outer membrane protein. To use this secretion system, you should remind to add HlyA C-terminal sequence to the molecule you wish to secret. HlyA connects to the complex HlyB-HlyD, which induces contact with TolC, creating a periplasmic export channel. | The promoter J23111 constitutively produces the alpha-hemolysin secretion system from Escherichia coli. The system is composed of HlyB, an internal membrane protein, HlyD, a membrane fusion protein, and TolC, an outer membrane protein. To use this secretion system, you should remind to add HlyA C-terminal sequence to the molecule you wish to secret. HlyA connects to the complex HlyB-HlyD, which induces contact with TolC, creating a periplasmic export channel. | ||
Latest revision as of 16:26, 9 December 2019
Alpha-hemolysin secretion system
This is a type I secretion system, used for protein translocation.
Usage and Biology
The promoter J23111 constitutively produces the alpha-hemolysin secretion system from Escherichia coli. The system is composed of HlyB, an internal membrane protein, HlyD, a membrane fusion protein, and TolC, an outer membrane protein. To use this secretion system, you should remind to add HlyA C-terminal sequence to the molecule you wish to secret. HlyA connects to the complex HlyB-HlyD, which induces contact with TolC, creating a periplasmic export channel.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1344
Illegal BglII site found at 2007
Illegal BamHI site found at 4051 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 274
Illegal AgeI site found at 3511
Illegal AgeI site found at 3568 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 3903