Difference between revisions of "Part:BBa K2918019"
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| − | A level 1 construct with a Wild-type T7 promoter, a universal RBS, phi29 TP and a T7 terminator | + | A level 1 MoClo construct with a Wild-type T7 promoter, a universal RBS, phi29 TP and a T7 terminator |
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Revision as of 23:07, 6 December 2019
WT T7 promoter - Universal RBS - Φ29 TP - T7 terminator
A level 1 MoClo construct with a Wild-type T7 promoter, a universal RBS, phi29 TP and a T7 terminator
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 237
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 557
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
The construct is confirmed by sequencing and there are no mutations.
Toxicity
Our Sci-Phi 29 tool is based on four components of the Φ29 bacteriophage: DNAP, TP, p5 and p6. However, overexpression of these proteins are toxic for the cell. In order to determine the optimal expression levels of the proteins in live cells, we carried out viability assays in E. coli BL21(DE3) pLysS. The results are shown in the graphs below.
