Difference between revisions of "Part:BBa K3063987"
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===Usage and Biology=== | ===Usage and Biology=== | ||
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| + | <span class='h3bb'>Sequence and Features</span> | ||
| + | <partinfo>BBa_K3063987 SequenceAndFeatures</partinfo> | ||
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| + | <!-- Uncomment this to enable Functional Parameter display | ||
| + | ===Functional Parameters=== | ||
| + | <partinfo>BBa_K3063987 parameters</partinfo> | ||
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__NOTOC__ | __NOTOC__ | ||
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=References= | =References= | ||
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Revision as of 10:54, 25 November 2019
eSIBR casette with amiRNA insertion site
eSIBR casette with amiRNA insertion site
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 585
Illegal BglII site found at 1409
Illegal XhoI site found at 1486 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1866
- 1000COMPATIBLE WITH RFC[1000]
The part encodes three amiRNAs that silence signal transducer and activator of transcription 3 (STAT3), SRY (sex determining region Y)-box 4 (SOX4) and SRY (sex determining region Y)-box 9 (SOX9). STAT3-, SOX4- and SOX9-targeting amiRNAs in the transformed salmonella, will form dsRNAs upon binding to stemness STAT3, SOX4 and SOX9 mRNAs in tumour cells respectively. Under the aid of this particular biobrick, silencing of STAT3, SOX4 and SOX9 genes in liver cancer stem cells can be achieved simultaneously.