Difference between revisions of "Part:BBa K3046021"

Latest revision as of 03:56, 22 October 2019

Promoter test plasmid pAMA1-zing_Pyr This plasmid is designed as a shuttle vector to test different fungal promoters

Usage and Biology

The pAMA1-zing-PyrG plasmid is able to replicate autonomously in Aspergillus niger due to its AMA1 sequence (BBa_K3046024).

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 3078
Illegal EcoRI site found at 5355
Illegal EcoRI site found at 9415
Illegal XbaI site found at 1257
Illegal XbaI site found at 2023
Illegal XbaI site found at 2505
Illegal XbaI site found at 3273
Illegal XbaI site found at 5160
Illegal XbaI site found at 5928
Illegal XbaI site found at 6410
Illegal SpeI site found at 1440
Illegal SpeI site found at 1451
Illegal SpeI site found at 1815
Illegal SpeI site found at 1947
Illegal SpeI site found at 2071
Illegal SpeI site found at 2178
Illegal SpeI site found at 2189
Illegal PstI site found at 4222
Illegal PstI site found at 10253
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 3078
Illegal EcoRI site found at 5355
Illegal EcoRI site found at 9415
Illegal SpeI site found at 1440
Illegal SpeI site found at 1451
Illegal SpeI site found at 1815
Illegal SpeI site found at 1947
Illegal SpeI site found at 2071
Illegal SpeI site found at 2178
Illegal SpeI site found at 2189
Illegal PstI site found at 4222
Illegal PstI site found at 10253
Illegal NotI site found at 1296
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 3078
Illegal EcoRI site found at 5355
Illegal EcoRI site found at 9415
Illegal BglII site found at 7082
Illegal BglII site found at 9054
Illegal BamHI site found at 9314
Illegal BamHI site found at 9440
Illegal BamHI site found at 9991
Illegal XhoI site found at 3619
Illegal XhoI site found at 4336
Illegal XhoI site found at 4814
Illegal XhoI site found at 9851
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 3078
Illegal EcoRI site found at 5355
Illegal EcoRI site found at 9415
Illegal XbaI site found at 1257
Illegal XbaI site found at 2023
Illegal XbaI site found at 2505
Illegal XbaI site found at 3273
Illegal XbaI site found at 5160
Illegal XbaI site found at 5928
Illegal XbaI site found at 6410
Illegal SpeI site found at 1440
Illegal SpeI site found at 1451
Illegal SpeI site found at 1815
Illegal SpeI site found at 1947
Illegal SpeI site found at 2071
Illegal SpeI site found at 2178
Illegal SpeI site found at 2189
Illegal PstI site found at 4222
Illegal PstI site found at 10253
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 3078
Illegal EcoRI site found at 5355
Illegal EcoRI site found at 9415
Illegal XbaI site found at 1257
Illegal XbaI site found at 2023
Illegal XbaI site found at 2505
Illegal XbaI site found at 3273
Illegal XbaI site found at 5160
Illegal XbaI site found at 5928
Illegal XbaI site found at 6410
Illegal SpeI site found at 1440
Illegal SpeI site found at 1451
Illegal SpeI site found at 1815
Illegal SpeI site found at 1947
Illegal SpeI site found at 2071
Illegal SpeI site found at 2178
Illegal SpeI site found at 2189
Illegal PstI site found at 4222
Illegal PstI site found at 10253
Illegal NgoMIV site found at 4356
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 6949
Illegal BsaI site found at 8128
Illegal BsaI.rc site found at 1484
Illegal BsaI.rc site found at 7902

Revision as of 01:41, 22 October 2019 (view source) Lookingforanswer (Talk \| contribs) ← Older edit		Latest revision as of 03:56, 22 October 2019 (view source) JMejlsted (Talk \| contribs)
Line 5:		Line 5:

	===Usage and Biology===		===Usage and Biology===
−	The pAMA1-zing-PyrG plasmid is able to replicate autonomously in <i>Aspergillus niger</i> due to its AMA1 sequence (BBa_K3046024). ~~Furthermore, the plasmid backbone (BBa_******)~~	+	The pAMA1-zing-PyrG plasmid is able to replicate autonomously in <i>Aspergillus niger</i> due to its AMA1 sequence (BBa_K3046024).