Difference between revisions of "Part:BBa K3098005"
 
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===Usage and Biology===
 
===Usage and Biology===
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Used to express bacteria cellulose synthase in E. coli. After adding IPTG to induce, the engineered bacteria will synthesize and secrete bacteria cellulose. '''Note that this part must be co-expressed with gene ''dgc'' (Diguanyl Cyclase[[Part:BBa_K3098003]]) to function.'''Considering the size of the gene, we didn't put gene ''gxcesAB'' and ''dgc'' together in one part. Double plasmids expression system (pQE-80L and pBAD33, for example) is recommended.  
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Used to express bacteria cellulose synthase in E. coli. After adding IPTG to induce, the engineered bacteria will synthesize and secrete bacteria cellulose. '''Note that this part must be co-expressed with gene ''dgc'' (Diguanyl Cyclase [[Part:BBa_K3098003]]) to function.''' Considering the size of the gene, we didn't put gene ''gxcesAB'' and ''dgc'' together in one part. Double plasmids expression system (pQE-80L and pBAD33, for example) is recommended.  
  
 
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Latest revision as of 03:50, 22 October 2019


Cellulose Synthase Operon with T5 promoter

Cellulose Synthase GxCesA (Part:BBa_K3098001) and GxCesB(Part:BBa_K3098002), with T5 promoter, two different Rbs and terminator.

Usage and Biology

Used to express bacteria cellulose synthase in E. coli. After adding IPTG to induce, the engineered bacteria will synthesize and secrete bacteria cellulose. Note that this part must be co-expressed with gene dgc (Diguanyl Cyclase Part:BBa_K3098003) to function. Considering the size of the gene, we didn't put gene gxcesAB and dgc together in one part. Double plasmids expression system (pQE-80L and pBAD33, for example) is recommended.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 655
    Illegal PstI site found at 817
    Illegal PstI site found at 2706
    Illegal PstI site found at 2715
    Illegal PstI site found at 3015
    Illegal PstI site found at 3555
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 655
    Illegal PstI site found at 817
    Illegal PstI site found at 2706
    Illegal PstI site found at 2715
    Illegal PstI site found at 3015
    Illegal PstI site found at 3555
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 655
    Illegal BglII site found at 1217
    Illegal BamHI site found at 4346
    Illegal XhoI site found at 307
    Illegal XhoI site found at 3729
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 655
    Illegal PstI site found at 817
    Illegal PstI site found at 2706
    Illegal PstI site found at 2715
    Illegal PstI site found at 3015
    Illegal PstI site found at 3555
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 655
    Illegal PstI site found at 817
    Illegal PstI site found at 2706
    Illegal PstI site found at 2715
    Illegal PstI site found at 3015
    Illegal PstI site found at 3555
    Illegal NgoMIV site found at 287
    Illegal AgeI site found at 2268
    Illegal AgeI site found at 4401
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4172
    Illegal BsaI.rc site found at 1792