Difference between revisions of "Part:BBa K3122008"
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<partinfo>BBa_K3122008 short</partinfo> | <partinfo>BBa_K3122008 short</partinfo> | ||
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| − | + | C-myc tag was added at the C-terminal site of ompT protein to allow subsequent detection by Western Blot, as no antibody has been developed against ompT yet. We designed a new scar compatible with the assembly for this purpose, as MoClo did not suggest anyone for C-terminal tags. Although cloning of this part has not been accomplished by our team because of the lack of ompT, we think this contribution will be rather useful for future teams aiming to perform MoClo assemblies. | |
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| − | < | + | <h2> Design </h2> |
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| + | The new scar added to iGEM type IIS standard is the following: | ||
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| + | Left tag scar: 5' AGCC 3' | ||
| + | Right tag scar: 5' GCTT 3' | ||
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| + | This scars allows positioning the tag at the C terminal site of the CDS protein. | ||
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<partinfo>BBa_K3122008 parameters</partinfo> | <partinfo>BBa_K3122008 parameters</partinfo> | ||
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Revision as of 03:44, 22 October 2019
cMyc-Tag (TypeIIS adapted)
C-myc tag was added at the C-terminal site of ompT protein to allow subsequent detection by Western Blot, as no antibody has been developed against ompT yet. We designed a new scar compatible with the assembly for this purpose, as MoClo did not suggest anyone for C-terminal tags. Although cloning of this part has not been accomplished by our team because of the lack of ompT, we think this contribution will be rather useful for future teams aiming to perform MoClo assemblies.
Design
The new scar added to iGEM type IIS standard is the following:
Left tag scar: 5' AGCC 3' Right tag scar: 5' GCTT 3'
This scars allows positioning the tag at the C terminal site of the CDS protein.