Difference between revisions of "Part:BBa K2380001"

Revision as of 03:36, 22 October 2019

Chitin synthase NodC with RBS and Anderson-Promoter

The Chitin Synthase (CHS) NodC from Rhizobium leguminosarum is a N-acetylglucosaminyl transferase, that catalyzes the formation of chitin oligomers by using UDP-acetylglucosamine as donor molecule and N-acetylglucosamine as acceptor. The expression of NodC is regulated by the constitutive Anderson-promoter system (BBa_K2380025). For further characterizations please visit BBa_K2380000 or our [http://2017.igem.org/Team:TU_Darmstadt/project/chitin_synthase wiki].

Plasmidcard

Characterization

The figure shown below shows NodC with an Anderson on the top and a negative control from the same plate on the bottom. The chitin product was shown to be produced since the cells fluoresce after being mixed with the calcofluor white assay. The control was used as a comparison to show how wildtype E. coli does not have most of its cells fluoresce. https://2019.igem.org/File:T--Purdue--G1comparison.jpg

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 36
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 438
1000
COMPATIBLE WITH RFC[1000]

@@ Line 9: / Line 9: @@
 <h2>Characterization</h2>
-To characterize this part Purdue iGEM performed a Calcofluor white stain. Calcofluor white fluoresces blue when in the presence of chitin and UV light. As seen in the image bellow, the sample with this part(top) fluoresced much brighter and much more than the control (bottom) that only contained a blank backbone.
+The figure shown below shows NodC with an Anderson on the top and a negative control from the same plate on the bottom. The chitin product was shown to be produced since the cells fluoresce after being mixed with the calcofluor white assay. The control was used as a comparison to show how wildtype E. coli does not have most of its cells fluoresce.
 https://2019.igem.org/File:T--Purdue--G1comparison.jpg