Difference between revisions of "Part:BBa K3149000"
Rafael elu (Talk | contribs) |
Rafael elu (Talk | contribs) |
||
| Line 2: | Line 2: | ||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K3149000 short</partinfo> | <partinfo>BBa_K3149000 short</partinfo> | ||
| − | + | <center> | |
<p>We designed a new part (pBAD+Trz1) to evaluate the influence of the overexpression of the Trz1 receptor (and the subsequent overstimulation of the endogenous EnvZ/OmpR system) in the growth kinetics of a wild type NEB-5 alpha strain. This construct will be cloned into the pSB1C3 vector and used to transform NEB-5 alpha | <p>We designed a new part (pBAD+Trz1) to evaluate the influence of the overexpression of the Trz1 receptor (and the subsequent overstimulation of the endogenous EnvZ/OmpR system) in the growth kinetics of a wild type NEB-5 alpha strain. This construct will be cloned into the pSB1C3 vector and used to transform NEB-5 alpha | ||
cells. We will then grow our transformants in different culture media to evaluate their growth kinetics at high glucose concentrations using a standard growth curve assay. | cells. We will then grow our transformants in different culture media to evaluate their growth kinetics at high glucose concentrations using a standard growth curve assay. | ||
</p> | </p> | ||
| − | + | https://2019.igem.org/wiki/images/d/d9/T--TecMonterrey_GDL--Results-7.png | |
<p>10kb: Quick-Load Purple 2-log DNA Ladder (10kb), M1: Miniprep NEB 5-alpha E. coli cells[pBAD-TRZ1], D1: NEB 5-alpha E. coli cells[pBAD-TRZ1] digested with ECORI-HF.</p> | <p>10kb: Quick-Load Purple 2-log DNA Ladder (10kb), M1: Miniprep NEB 5-alpha E. coli cells[pBAD-TRZ1], D1: NEB 5-alpha E. coli cells[pBAD-TRZ1] digested with ECORI-HF.</p> | ||
| − | + | </center> | |
| − | + | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 03:25, 22 October 2019
TRZ1 receptor under the control of the inducible pBAD (strong) promoter
We designed a new part (pBAD+Trz1) to evaluate the influence of the overexpression of the Trz1 receptor (and the subsequent overstimulation of the endogenous EnvZ/OmpR system) in the growth kinetics of a wild type NEB-5 alpha strain. This construct will be cloned into the pSB1C3 vector and used to transform NEB-5 alpha cells. We will then grow our transformants in different culture media to evaluate their growth kinetics at high glucose concentrations using a standard growth curve assay.
10kb: Quick-Load Purple 2-log DNA Ladder (10kb), M1: Miniprep NEB 5-alpha E. coli cells[pBAD-TRZ1], D1: NEB 5-alpha E. coli cells[pBAD-TRZ1] digested with ECORI-HF.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 65
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 503
- 1000COMPATIBLE WITH RFC[1000]