Difference between revisions of "Part:BBa K3149000"

 
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<partinfo>BBa_K3149000 short</partinfo>
 
<partinfo>BBa_K3149000 short</partinfo>
  
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For the mathematical model we decide to work with the growth of the bacteria in different  
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<p>We designed a new part (pBAD+Trz1) to evaluate the influence of the overexpression of the Trz1 receptor (and the subsequent overstimulation of the endogenous EnvZ/OmpR system) in the growth kinetics of a wild type NEB-5 alpha strain. This construct will be cloned into the pSB1C3 vector and used to transform NEB-5 alpha
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The characterization of an existent part, a promoter which is Pompc with a fluorescent reporter that is GFP is presented.
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cells. We will then grow our transformants in different culture media to evaluate their growth kinetics at high glucose concentrations using a standard growth curve assay.
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</p>
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<img src='https://2019.igem.org/wiki/images/d/d9/T--TecMonterrey_GDL--Results-7.png'>
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<p>10kb: Quick-Load Purple 2-log DNA Ladder (10kb), M1: Miniprep NEB 5-alpha E. coli cells[pBAD-TRZ1], D1: NEB 5-alpha E. coli cells[pBAD-TRZ1] digested with ECORI-HF.</p>
  
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Various features of the project have been modelled to verify the validity of our proposed solution and to enable us to make predictions based on different
 
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aspects of the system.
 
  
  

Revision as of 03:24, 22 October 2019


TRZ1 receptor under the control of the inducible pBAD (strong) promoter

We designed a new part (pBAD+Trz1) to evaluate the influence of the overexpression of the Trz1 receptor (and the subsequent overstimulation of the endogenous EnvZ/OmpR system) in the growth kinetics of a wild type NEB-5 alpha strain. This construct will be cloned into the pSB1C3 vector and used to transform NEB-5 alpha cells. We will then grow our transformants in different culture media to evaluate their growth kinetics at high glucose concentrations using a standard growth curve assay.

<img src='https://2019.igem.org/wiki/images/d/d9/T--TecMonterrey_GDL--Results-7.png'>

10kb: Quick-Load Purple 2-log DNA Ladder (10kb), M1: Miniprep NEB 5-alpha E. coli cells[pBAD-TRZ1], D1: NEB 5-alpha E. coli cells[pBAD-TRZ1] digested with ECORI-HF.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 503
  • 1000
    COMPATIBLE WITH RFC[1000]