Difference between revisions of "Part:BBa K2916011"

Revision as of 03:23, 22 October 2019

MK protein equipped with a 6x HIS affinity tag

Adenylate kinase (Myokinase) is a protein that is used in the PURE and OnePot PURE cell-free systems.

Used in OnePot PURE In our part, besides the sequence encoding for the protein we also have a hexahistidine-tag to allow us purify the protein.

All the data regarding the characterization of that part can be found in the composite part: BBa_K2916046 .

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 583
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 403
Illegal BsaI.rc site found at 433

@@ Line 11: / Line 11: @@
 Used in OnePot PURE
+In our part, besides the sequence encoding for the protein we also have a hexahistidine-tag to allow us purify the protein.
+===Characterization===
+All the data regarding the characterization of that part can be found in the composite part: <html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part:BBa_K2916046"> BBa_K2916046 </a></html>.
-<!-- -->
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K2916011 SequenceAndFeatures</partinfo>