Difference between revisions of "Part:BBa K3059509:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | In the system designed by Karig et al, dsRed-express serves as a reporter to indicate that the first signalling pathway has been activated, leading to production of the activator and inhibitor signals. | |
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===Source=== | ===Source=== | ||
Latest revision as of 03:20, 22 October 2019
dsRed-exp α_H - sequence confirmed
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 388
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 388
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 388
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 388
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 41
Illegal BsaI.rc site found at 735
Design Notes
In the system designed by Karig et al, dsRed-express serves as a reporter to indicate that the first signalling pathway has been activated, leading to production of the activator and inhibitor signals.
Source
Synthesized via IDT; used designer primers to add Pad sequences, BsaI cutsites, and sticky ends.
References
1. Karig, D., Martini, K. M., Lu, T., Delateur, N. A., Goldenfeld, N., & Weiss, R. (2018). Stochastic Turing patterns in a synthetic bacterial population. Proceedings of the National Academy of Sciences, 115(26), 6572-6577. doi:10.1073/pnas.1720770115
2. Karig, D. K. (2007). Engineering multi-signal synthetic biological systems. (Doctoral dissertation). Princeton University, USA.