Difference between revisions of "Part:BBa K3122006"

 
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<partinfo>BBa_K3122006 short</partinfo>
 
<partinfo>BBa_K3122006 short</partinfo>
  
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Ptac, trp and lac regulated promoter (Part:BBa_K864400), adapted to MoClo standard by our team. It was designed to control Streptavidin-CBDs expression via IPTG induction.
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===Usage and Biology===
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<h2> Description </h2>
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3122006 SequenceAndFeatures</partinfo>
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Promotor Ptac is a combination of the different promoters from the lac and tpr operons. The advantages of this promoter include its high efficiency (11 times more than the lac one and 3 times more than the tpr one) and the fine control of the expression it allows (it can be repressed by lac repressor and derepressed with isopropyl-beta-D-thiogalactoside).
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<h2> Characterization </h2>
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Characterization of this part was planned to be conducted by building three transcriptional units (<partinfo>BBa_K3122017</partinfo>, <partinfo>BBa_K3122018</partinfo>, <partinfo>BBa_K31220179</partinfo>) expressing different recombinant proteins. Unfortunately, no results were obtained.
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<h2> References </h2>
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de Boer, H., Comstock, L. and Vasser, M. (1983). The tac promoter: a functional hybrid derived from the trp and lac promoters. Proceedings of the National Academy of Sciences, 80(1), pp.21-25.
  
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===Functional Parameters===
 
 
<partinfo>BBa_K3122006 parameters</partinfo>
 
<partinfo>BBa_K3122006 parameters</partinfo>
 
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Revision as of 03:14, 22 October 2019


Inducible Ptac promoter (TypeIIS adapted)


Ptac, trp and lac regulated promoter (Part:BBa_K864400), adapted to MoClo standard by our team. It was designed to control Streptavidin-CBDs expression via IPTG induction.

Description

Promotor Ptac is a combination of the different promoters from the lac and tpr operons. The advantages of this promoter include its high efficiency (11 times more than the lac one and 3 times more than the tpr one) and the fine control of the expression it allows (it can be repressed by lac repressor and derepressed with isopropyl-beta-D-thiogalactoside).

Characterization

Characterization of this part was planned to be conducted by building three transcriptional units (BBa_K3122017, BBa_K3122018, No part name specified with partinfo tag.) expressing different recombinant proteins. Unfortunately, no results were obtained.


References

de Boer, H., Comstock, L. and Vasser, M. (1983). The tac promoter: a functional hybrid derived from the trp and lac promoters. Proceedings of the National Academy of Sciences, 80(1), pp.21-25.