Difference between revisions of "Part:BBa K2957003"

Revision as of 03:09, 22 October 2019

hEF1a, IL-8 (CXCL-8)

Contents: hEF1a, IL-8 (CXCL-8), SynthpA, with Carb resistance

What is it?: A Composite part; it is a genetic circuit with hEF1a promoter and IL-8 gene allowing for constitutive IL-8 expression

What does it do?: It codes for the chemokine, IL-8 (CXCL8), which is involved in an inflammatory response. It was important for our project in particular because of its involvement in the immune system and is known to create neutrophil swarming.

How to use it?: This part was used for Type IIS Cloning and transfected into HEKs. We then were able to test for secretion of IL8. See more on the Parts Overview Page or MIT iGEM 2019 wiki: https://2019.igem.org/Team:MIT/Parts.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 567
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 485
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 567
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 567
Illegal AgeI site found at 81
Illegal AgeI site found at 573
1000
COMPATIBLE WITH RFC[1000]

@@ Line 11: / Line 11: @@
 <b>How to use it?</b>: This part was used for Type IIS Cloning and transfected into HEKs. We then were able to test for secretion of IL8. See more on the Parts Overview Page or MIT iGEM 2019 wiki: https://2019.igem.org/Team:MIT/Parts.
-<img src = "https://2019.igem.org/wiki/images/8/8a/T--MIT----hef1a-IL8.png" >
+https://static.igem.org/mediawiki/parts/5/50/T--MIT----SecretionTest.png
 <!-- Add more about the biology of this part here