Difference between revisions of "Part:BBa K2944001"

Latest revision as of 03:08, 22 October 2019

Glucose oxidase optimized for Saccharomyces cerevisiae

Glucose oxidase from Aspergillus niger was codon optimized for transcription in Saccharomyces cerevisiae. Glucose oxidase catalyzes the oxidation of glucose to D-glucono-1,5-lactone and hydrogen peroxide. Original sequence was obtained from BBa_K2238000 and then codon optimized, but retains the His₆-tag for purification.

This part is also flanked by assembly scars on both sides, between the promoter and the gene (5'-agatct-3') and as well between the gene and the terminator (5'-atcctaactcgag-3')

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1
Illegal BamHI site found at 42
Illegal BamHI site found at 352
Illegal XhoI site found at 1916
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 376
Illegal BsaI.rc site found at 1594

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2944001 short</partinfo>
-Glucose oxidase from Aspergillus niger was codon optimized for transcription in Saccharomyces cerevisiae. Glucose oxidase catalyzes the oxidation of glucose to D-glucono-1,5-lactone and hydrogen peroxide. Original sequence was obtained from BBa_K2238000 and then codon optimized, but retains the His6-tag for purification.
+Glucose oxidase from Aspergillus niger was codon optimized for transcription in <i>Saccharomyces cerevisiae</i>. Glucose oxidase catalyzes the oxidation of glucose to D-glucono-1,5-lactone and hydrogen peroxide. Original sequence was obtained from BBa_K2238000 and then codon optimized, but retains the His<sub>6</sub>-tag for purification.
+This part is also flanked by assembly scars on both sides, between the promoter and the gene (5'-agatct-3') and as well between the gene and the terminator (5'-atcctaactcgag-3')
 <!-- Add more about the biology of this part here