Difference between revisions of "Part:BBa K3046031"

 
 
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This part is designed to be inserted into pSB1C3 by Gibson cloning to make an integration plasmid for Aspergillus niger. The part is meant to be linearised by PCR prior to protoplast-mediated transformation, where the IS2 sites will lead to genomic integration of the insert (in this case just PyrG part BBa_K3046022) into the albA conidial pigment gene. Correct gene integration will therefore yield white/yellow conidia instead of the black conidia seen for the wildtype A. niger.
 
This part is designed to be inserted into pSB1C3 by Gibson cloning to make an integration plasmid for Aspergillus niger. The part is meant to be linearised by PCR prior to protoplast-mediated transformation, where the IS2 sites will lead to genomic integration of the insert (in this case just PyrG part BBa_K3046022) into the albA conidial pigment gene. Correct gene integration will therefore yield white/yellow conidia instead of the black conidia seen for the wildtype A. niger.
  
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The plasmid contains a GFP reporter, that is being controlled by the constitutive PTrpC promoter. This part has *******
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The plasmid contains a GFP reporter, that is being controlled by the constitutive PTrpC promoter.  
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 03:02, 22 October 2019


pPEA2P1 insert

This part is designed to be inserted into pSB1C3 by Gibson cloning to make an integration plasmid for Aspergillus niger. The part is meant to be linearised by PCR prior to protoplast-mediated transformation, where the IS2 sites will lead to genomic integration of the insert (in this case just PyrG part BBa_K3046022) into the albA conidial pigment gene. Correct gene integration will therefore yield white/yellow conidia instead of the black conidia seen for the wildtype A. niger.

The plasmid contains a GFP reporter, that is being controlled by the constitutive PTrpC promoter.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1858
    Illegal EcoRI site found at 3709
    Illegal EcoRI site found at 5145
    Illegal XbaI site found at 1623
    Illegal XbaI site found at 3740
    Illegal SpeI site found at 1097
    Illegal PstI site found at 893
    Illegal PstI site found at 4547
    Illegal PstI site found at 6289
    Illegal PstI site found at 6890
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1858
    Illegal EcoRI site found at 3709
    Illegal EcoRI site found at 5145
    Illegal NheI site found at 2483
    Illegal SpeI site found at 1097
    Illegal PstI site found at 893
    Illegal PstI site found at 4547
    Illegal PstI site found at 6289
    Illegal PstI site found at 6890
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1858
    Illegal EcoRI site found at 3709
    Illegal EcoRI site found at 5145
    Illegal BglII site found at 3342
    Illegal BglII site found at 6387
    Illegal BamHI site found at 1729
    Illegal BamHI site found at 3602
    Illegal BamHI site found at 3734
    Illegal BamHI site found at 4285
    Illegal XhoI site found at 548
    Illegal XhoI site found at 735
    Illegal XhoI site found at 963
    Illegal XhoI site found at 4145
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1858
    Illegal EcoRI site found at 3709
    Illegal EcoRI site found at 5145
    Illegal XbaI site found at 1623
    Illegal XbaI site found at 3740
    Illegal SpeI site found at 1097
    Illegal PstI site found at 893
    Illegal PstI site found at 4547
    Illegal PstI site found at 6289
    Illegal PstI site found at 6890
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1858
    Illegal EcoRI site found at 3709
    Illegal EcoRI site found at 5145
    Illegal XbaI site found at 1623
    Illegal XbaI site found at 3740
    Illegal SpeI site found at 1097
    Illegal PstI site found at 893
    Illegal PstI site found at 4547
    Illegal PstI site found at 6289
    Illegal PstI site found at 6890
    Illegal NgoMIV site found at 6277
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3126
    Illegal SapI site found at 6778
    Illegal SapI site found at 7087