Difference between revisions of "Part:BBa K3304003"

Revision as of 02:54, 22 October 2019

Reporter complex for output - TCC

This is the reporter complex that generates the fluorescent output to be measured as a quantitative readout of the formal CRN's function. The top strand is labeled with a fluorophore molecule at its 3' end while it's complementary strand is labeled with a quencher at it's 5' end. The quencher is able to absorb the fluorescence emitted by the fluorophore for as long as they're paired. The strand displacement reaction induces the separation of the quencher from the fluorophore producing measurable output.

https://2019.igem.org/File:T--Thessaloniki--results-f1.png The voltage diagram from our MOSFET circuit that translates the binding of a single-stranded DNA to the bottom strand of the reporter complex that is probed to a DNA plated electrode. The binding is expressed through the change in capacitance and it is viewed as drop-in voltage readout. As a bigger concentration of the corresponding strand is bound to the DNA probes we observe a bigger dip in voltage https://2019.igem.org/File:T--Thessaloniki--results-f1.png https://parts.igem.org/File:BBa_K3304003-ReporterCharacterizationThessaloniki2019.png

Sequence and Features