Difference between revisions of "Part:BBa K2074026"
 
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===Usage and Biology===
 
===Usage and Biology===
  
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2074026 SequenceAndFeatures</partinfo>
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[[File:T--FAFU-CHINA--E.png|600px|thumb|center|'''Figure.1''' The gel electrophoresis result of EGFP]]
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===Functional Parameters===
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<partinfo>BBa_K2074026 AddReview number</partinfo>
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<partinfo>BBa_K2074026 parameters</partinfo>
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<I>Username</I>
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Enter the review inofrmation here.
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<partinfo>BBa_I0500 AddReview 5</partinfo>
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<I><b>[https://2019.igem.org/Team:FAFU-CHINA 2019 iGEM team FAFU-CHINA]</B></I>
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[https://2019.igem.org/Team:FAFU-CHINA 2019 iGEM team FAFU-CHINA] The Hsp70A-RbcS2 promoter enhances the expression of EGFP after heat shock initiation. It is seen that the FAFU-CHINA team in 2016 made Western blost on the expression of EGFP, at a temperature of 40 degrees Celsius. Heat shock 0, 80, 60, 40, 20min.
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This year (2019), we used the same promoter and EGFP for QPCR quantification, provided that the temperature was 40 ° C, heat shock 0, 40, 80, 100, 120, 140 min. The specific data we have obtained is the result of the following tables and images:
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[[Image:HeatShockTime.png|left|thumb|400px|'''Heat Shock Time(min).''']]
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[[Image:T--FAFU-CHINA--EGFP.png|left|thumb|400px|'''EGFP characterization data.''']]
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The results showed that after 0, 40, 80 min, the results were the same as the 2016 FAFU-CHINA results. The new supplement condition we have this year is that the heat shock is 100, 120, 140min. The experimental results show that the relative expression of EGFP is increasing under these conditions, but the results are similar at 120 and 140 min, this may be the limit of the conditions that enhance EGFP expression.
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Latest revision as of 02:51, 22 October 2019


EGFP(Codon optimization)

Our part is coding the EGFP,We transferred the gene EGFP into plasmid ,and transformed the recombinant plasmid into Chlamydomonas reinhardti to express protein and to test whether our systerm work or not. We used the promoter to initiate expression of EGFP, resulting in promoter regulation of gene expression data

The efficiency of Hsp70A-Rbc S2 promoter can be improved when environmental temperature is larger than 40℃. It means that the toxins express device will express more toxins in summer closed waters.The closed waters usually is larvae living place. The temperature in the open water will be much lower than this value. This decided would reduce the amount of pro-toxic protein present in the non-functioning waters.

T--FAFU-CHINA--Result4.png
T--FAFU-CHINA--Result5.png
T--FAFU-CHINA--Result6.png

Alignment and transition:

T--FAFU-CHINA--EGFPoptimization.png
T--FAFU-CHINA--EGFPoptimization2.png


UNIQ154e5a415056c6e6-partinfo-00000001-QINU

•••••

2019 iGEM team FAFU-CHINA

2019 iGEM team FAFU-CHINA The Hsp70A-RbcS2 promoter enhances the expression of EGFP after heat shock initiation. It is seen that the FAFU-CHINA team in 2016 made Western blost on the expression of EGFP, at a temperature of 40 degrees Celsius. Heat shock 0, 80, 60, 40, 20min. This year (2019), we used the same promoter and EGFP for QPCR quantification, provided that the temperature was 40 ° C, heat shock 0, 40, 80, 100, 120, 140 min. The specific data we have obtained is the result of the following tables and images:


Heat Shock Time(min).
EGFP characterization data.

The results showed that after 0, 40, 80 min, the results were the same as the 2016 FAFU-CHINA results. The new supplement condition we have this year is that the heat shock is 100, 120, 140min. The experimental results show that the relative expression of EGFP is increasing under these conditions, but the results are similar at 120 and 140 min, this may be the limit of the conditions that enhance EGFP expression.

UNIQ154e5a415056c6e6-partinfo-00000003-QINU