Difference between revisions of "Part:BBa K2916008"

Revision as of 02:45, 22 October 2019

EF-G protein equipped with a 6x HIS affinity tag

Elongation Factor G is a protein that is used in the PURE and OnePot PURE cell-free systems.

Usage and Biology

Elongation factors are a set of proteins that function during the second step of protein biosynthesis, the elongation phase. They act upon the ribosome, to facilitate translational elongation from formation of the first to the last peptide bond of a growing polypeptide. The elongation phase is the most rapid step in translation, so the factors also regulate the procedure happening during that step to ensure high accuracy of the translation.

The Elongation Factor G in particular catalyzes the translocation of the tRNA and mRNA down the ribosome at the end of each round of polypeptide elongation.

In our part, besides the sequence encoding for the protein we also have a hexahistidine-tag to allow us purify the protein.

For our project we used EF-G as a part of the protein solution needed for  OnePot PURE cell-free system  using the method of gravity flow affinity chromatography, as described in the  protocol  we designed.

Sequence and Features