Difference between revisions of "Part:BBa K1758331"
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<h2><b>IISER Pune 2019</b> - Characterisation</h2>
 
<h2><b>IISER Pune 2019</b> - Characterisation</h2>
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<p>To check for the activity of the promoter in this construct, fluorescence measurements of cells transformed with the biobrick were taken in different conditions. </p>
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<p>DH5 alpha cells transformed with the biobrick were cultured in LB and M9 media and their fluorescence measurements were taken either 12 or 16 hours from the time of inoculation. These data was subsequently compared with the fluorescence data of a negative control (DH5 alpha cells producing no fluorescent proteins) and a positive control (DH5 alpha cells consecutively expressing RFP, transformed with BBa_J04450)</p>
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<p></p>

Revision as of 02:35, 22 October 2019


Lead responsive promoter with mRFP

lead responsive promoter with mRFP for detection

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


IISER Pune 2019 - Characterisation

To check for the activity of the promoter in this construct, fluorescence measurements of cells transformed with the biobrick were taken in different conditions.

DH5 alpha cells transformed with the biobrick were cultured in LB and M9 media and their fluorescence measurements were taken either 12 or 16 hours from the time of inoculation. These data was subsequently compared with the fluorescence data of a negative control (DH5 alpha cells producing no fluorescent proteins) and a positive control (DH5 alpha cells consecutively expressing RFP, transformed with BBa_J04450)