Difference between revisions of "Part:BBa K3093003"

Revision as of 02:34, 22 October 2019

chbB promoter with a new -35

ChbB promotor is the promotor of chitobiose operon. It has the NagC, ChbR and CAP binding sites and can be activated by N -acetylglucosamine disaccharide, chitobiose.

The chbB promotor was obtained from E.coli MG1655 through PCR. And we remove the strong NagC binding site and change the old -35 signal "TACTAT" to the new -35 signal "TTGACA"

References

[1]Plumbridge J, Pellegrini O. Expression of the chitobiose operon of Escherichia coli is regulated by three transcription factors: NagC, ChbR and CAP. Mol Microbiol. 2004 Apr;52(2):437-49. PubMed PMID: 15066032.

[2]Kachroo AH, Kancherla AK, Singh NS, Varshney U, Mahadevan S. Mutations that alter the regulation of the chb operon of Escherichia coli allow utilization of cellobiose. Mol Microbiol. 2007 Dec;66(6):1382-95. Epub 2007 Nov 19. PubMed PMID: 18028317.

[3]Joseph AM, Sonowal R, Mahadevan S. A comparative study of the evolution of cellobiose utilization in Escherichia coli and Shigella sonnei. Arch Microbiol. 2017 Mar;199(2):247-257. doi: 10.1007/s00203-016-1299-0. Epub 2016 Oct 1. PubMed PMID: 27695910.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 ChbB promotor is the promotor of chitobiose operon. It has the NagC, ChbR and CAP binding sites and can be activated by N -acetylglucosamine disaccharide, chitobiose.
+The chbB promotor was obtained from E.coli MG1655 through PCR. And we remove the strong NagC binding site and change the old -35 signal "TACTAT" to the new -35 signal "TTGACA"
+===References===
+[1]Plumbridge J, Pellegrini O. Expression of the chitobiose operon of Escherichia coli is regulated by three transcription factors: NagC, ChbR and CAP. Mol Microbiol. 2004 Apr;52(2):437-49. PubMed PMID: 15066032.
+[2]Kachroo AH, Kancherla AK, Singh NS, Varshney U, Mahadevan S. Mutations that alter the regulation of the chb operon of Escherichia coli allow utilization of cellobiose. Mol Microbiol. 2007 Dec;66(6):1382-95. Epub 2007 Nov 19. PubMed PMID: 18028317.
+[3]Joseph AM, Sonowal R, Mahadevan S. A comparative study of the evolution of cellobiose utilization in Escherichia coli and Shigella sonnei. Arch Microbiol. 2017 Mar;199(2):247-257. doi: 10.1007/s00203-016-1299-0. Epub 2016 Oct 1. PubMed PMID: 27695910.
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