Difference between revisions of "Part:BBa K3192019:Design"

 
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
na
+
<p>This part DNA sequence was taken from NCBI and codon optimized for <i>E. coli</i> K12 chassis. The sequence was then reviewed for any illegal restriction sites and conservative edits were made as necessary. The coding sequence was then designed for Golden Gate (using Teselagen Software) assembly and then synthesized. This part was assembled into a larger composite part using Golden Gate Assembly and then sequenced for identity confirmation. </p>
  
  
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===Source===
 
===Source===
  
−
Pseudomonas putida
+
<i>Pseudomonas putida</i>
  
 
===References===
 
===References===
 +
<p><i>Pseudomonas putida</i> influx porin (styE) gene, complete cds - Nucleotide - NCBI. (n.d.). Retrieved from https://www.ncbi.nlm.nih.gov/nuccore/AY450871.1.</p>

Latest revision as of 02:29, 22 October 2019


styE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1040
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 125


Design Notes

This part DNA sequence was taken from NCBI and codon optimized for E. coli K12 chassis. The sequence was then reviewed for any illegal restriction sites and conservative edits were made as necessary. The coding sequence was then designed for Golden Gate (using Teselagen Software) assembly and then synthesized. This part was assembled into a larger composite part using Golden Gate Assembly and then sequenced for identity confirmation.


Source

Pseudomonas putida

References

Pseudomonas putida influx porin (styE) gene, complete cds - Nucleotide - NCBI. (n.d.). Retrieved from https://www.ncbi.nlm.nih.gov/nuccore/AY450871.1.