Difference between revisions of "Part:BBa K3046019"
 
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===Usage and Biology===
 
===Usage and Biology===
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This secretion tag was predicted by the DeepLoc-1.0 software [1] to facilitate the export of mCherry  
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This secretion tag was predicted by the DeepLoc-1.0 software [1] to facilitate the export of mCherry.
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<br>
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When analyzed with DeepLoc-1.0, the following results were shown when this secretion tag is either absent or present.
  
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<figure>
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<img src="https://static.igem.org/mediawiki/parts/f/f9/T--DTU-Denmark--mCherry_with_tag.png" style="width: 50%; padding: 5px;" >
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<figcaption> Figure 1: The figure the sub-cellular location as predicted by DeepLoc-1.0 is <b> the cytoplasm</b> if this protein secretion tag is not attached to mCherry (BBa_J06504).
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</figcaption>
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</figure>
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<br>
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<figure>
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<img src="https://static.igem.org/mediawiki/parts/5/52/T--DTU-Denmark--mCherry_no_tag.png" style="width: 50%; padding: 5px;" >
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<figcaption> Figure 2: The figure the sub-cellular location as predicted by DeepLoc-1.0 is <b>extracellular</b> if this protein secretion tag is attached to the N-terminus of mCherry (BBa_J06504).
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</figcaption>
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</figure>
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</html>
  
 
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Latest revision as of 02:25, 22 October 2019


Export signal from A. niger GlaA protein

Protein secretion tag from the GlaA enzyme from Aspergillus niger.


Usage and Biology

This secretion tag was predicted by the DeepLoc-1.0 software [1] to facilitate the export of mCherry.
When analyzed with DeepLoc-1.0, the following results were shown when this secretion tag is either absent or present.

Figure 1: The figure the sub-cellular location as predicted by DeepLoc-1.0 is the cytoplasm if this protein secretion tag is not attached to mCherry (BBa_J06504).

Figure 2: The figure the sub-cellular location as predicted by DeepLoc-1.0 is extracellular if this protein secretion tag is attached to the N-terminus of mCherry (BBa_J06504).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Functional Parameters

uniprotA2QHE1


[1]: Jose Juan Almagro Armenteros, Casper Kaae Sønderby, Søren Kaae Sønderby, Henrik Nielsen, Ole Winther; DeepLoc: prediction of protein subcellular localization using deep learning, Bioinformatics