Difference between revisions of "Part:BBa K3002232"

 
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<partinfo>BBa_K3002232 short</partinfo>
 
<partinfo>BBa_K3002232 short</partinfo>
  
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This composite part contains a spectinomycin resistance (BBa_K3002102) and the Mutate M from iGEM16_TJUSLS_China with the secretion signal cCA (BBa_K3002100) fused with an SP20HA-tag for easy detection via HA-antibody and enhanced secretion.
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This composite part contains a spectinomycin resistance (<a href="https://parts.igem.org/Part:BBa_K3002102">BBa_K3002102</a>) and the Mutate M from iGEM16_TJUSLS_China with the secretion signal cCA (<a href="https://parts.igem.org/Part:BBa_K3002100">BBa_K3002100</a>) fused with an SP20HA-tag for easy detection via HA-antibody and enhanced secretion.
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</html>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 02:25, 22 October 2019


L2 spectinomycin resistance + cCA_MutateM_SP20HA

This composite part contains a spectinomycin resistance (BBa_K3002102) and the Mutate M from iGEM16_TJUSLS_China with the secretion signal cCA (BBa_K3002100) fused with an SP20HA-tag for easy detection via HA-antibody and enhanced secretion.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2401
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2401
    Illegal NheI site found at 2665
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2401
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2401
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2401
    Illegal NgoMIV site found at 1401
    Illegal NgoMIV site found at 1584
    Illegal NgoMIV site found at 1694
    Illegal NgoMIV site found at 4325
  • 1000
    COMPATIBLE WITH RFC[1000]