Difference between revisions of "Part:BBa K3192018:Design"

 
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
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na
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<p>This part DNA sequence was taken from NCBI and codon optimized for <i>E. coli</i> K12 chassis. The sequence was then reviewed for any illegal restriction sites and conservative edits were made as necessary. The coding sequence was then designed for Golden Gate (using Teselagen Software) assembly and then synthesized. This part was assembled into a larger composite part using Golden Gate Assembly and then sequenced for identity confirmation. </p>
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+
  
  
 
===Source===
 
===Source===
  
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Pseudomas putida
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<i>Pseudomas putida</i>
  
 
===References===
 
===References===
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<p><i> Pseudomonas putida</i> strain SN1 StyA (styA), StyB (styB), StyC (styC), a - Nucleotide - NCBI. (n.d.). Retrieved from https://www.ncbi.nlm.nih.gov/nuccore/DQ177365.1.</p>

Latest revision as of 02:23, 22 October 2019


styD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1380
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 540
    Illegal NgoMIV site found at 907
    Illegal AgeI site found at 730
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1318


Design Notes

This part DNA sequence was taken from NCBI and codon optimized for E. coli K12 chassis. The sequence was then reviewed for any illegal restriction sites and conservative edits were made as necessary. The coding sequence was then designed for Golden Gate (using Teselagen Software) assembly and then synthesized. This part was assembled into a larger composite part using Golden Gate Assembly and then sequenced for identity confirmation.


Source

Pseudomas putida

References

Pseudomonas putida strain SN1 StyA (styA), StyB (styB), StyC (styC), a - Nucleotide - NCBI. (n.d.). Retrieved from https://www.ncbi.nlm.nih.gov/nuccore/DQ177365.1.