Difference between revisions of "Part:BBa K3002234"

 
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<partinfo>BBa_K3002234 short</partinfo>
 
<partinfo>BBa_K3002234 short</partinfo>
  
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This composite part contains a hygromycin resistance (BBa_K3002127) and the WT-PETase (BBa_K3002109) fused with an SP20HA-tag for easy detectionvia HA-antibody and enhanced secretion.
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<html>
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This composite part contains a hygromycin resistance (<a href="https://parts.igem.org/Part:BBa_K3002127">BBa_K3002127</a>) and the WT-PETase (<a href="https://parts.igem.org/Part:BBa_K3002109">BBa_K3002109</a>) fused with an SP20HA-tag for easy detectionvia HA-antibody and enhanced secretion.
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</html>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 02:21, 22 October 2019


L2 hygromycin resistance 2 + cCA_Mut-PETase_SP20HA

This composite part contains a hygromycin resistance (BBa_K3002127) and the WT-PETase (BBa_K3002109) fused with an SP20HA-tag for easy detectionvia HA-antibody and enhanced secretion.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 3453
    Illegal PstI site found at 4426
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2633
    Illegal PstI site found at 3453
    Illegal PstI site found at 4426
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 3453
    Illegal PstI site found at 4426
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 3453
    Illegal PstI site found at 4426
    Illegal NgoMIV site found at 1270
    Illegal NgoMIV site found at 1452
    Illegal NgoMIV site found at 3188
    Illegal NgoMIV site found at 3215
    Illegal NgoMIV site found at 4986
  • 1000
    COMPATIBLE WITH RFC[1000]