Difference between revisions of "Part:BBa K3259005"

Latest revision as of 02:21, 22 October 2019

composite part for Turing Pattern controlled by temperature

This part was assembled based on a paper (1) on the replication of Turing patterns by synthetic biology. In the formation of Turing pattern, substances called activators and inhibitors play a major role.

In this circuit, LasI and RhlI which works as activator and inhibitor respectively are both controlled by pLas-OR. PAI2 produced by LasI gene promotes the expression of both LasI and RhlI by binding to LasR. This makes PAI1 to be function as an activator for this Turing circuit. Conversely, RhlI produces PAI2 and binds to RhlR to promote the expression of CI. This results in suppression of RhlI and LasI expression, therefore PAI2 works as an inhibitor in this Turing pattern circuit. Turing pattern is generated by this gene circuit by the interaction between these two substances, namely PAI1 and PAI2.

Furthermore, since BBa_K3259001 part is also included into this gene circuit, it is expected that the pattern can be controlled by changes in temperature.

If you wish to know more about how Turing patterns are created, please read our Wiki (https://2019.igem.org/Team:TokyoTech/Experiments) Or read the paper in (1).

(1)David Karig, K. Michael Martini, Ting Lu, Nicholas A. DeLateur, Nigel Goldenfeld, and Ron Weiss (2018). Stochastic Turing patterns in a synthetic bacterial population . Proceedings of the National Academy of Sciences ,115(26), 6572-6577

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 58
Illegal NheI site found at 1120
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1917
Illegal BamHI site found at 3676
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 516
Illegal AgeI site found at 713
Illegal AgeI site found at 1479
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 4151
Illegal BsaI.rc site found at 6079

@@ Line 6: / Line 6: @@
 In this circuit, LasI and RhlI which works as activator and inhibitor respectively are both controlled by pLas-OR.
-PAI2 produced by LasI gene promotes the expression of both LasI and RhlI by binding to LasR. This makes PAI2 to be function as an activator for this Turing circuit.
+PAI2 produced by LasI gene promotes the expression of both LasI and RhlI by binding to LasR. This makes PAI1 to be function as an activator for this Turing circuit.
-Conversely, RhlI produces PAI1 and binds to RhlR to promote the expression of CI. This results in suppression of RhlI and LasI expression, therefore PAI1 works as an inhibitor in this Turing pattern circuit.
+Conversely, RhlI produces PAI2 and binds to RhlR to promote the expression of CI. This results in suppression of RhlI and LasI expression, therefore PAI2 works as an inhibitor in this Turing pattern circuit.
-Turing pattern is generated by this gene circuit by the interaction between these two substances, namely PAI2 and PAI1.
+Turing pattern is generated by this gene circuit by the interaction between these two substances, namely PAI1 and PAI2.
 Furthermore, since BBa_K3259001 part is also included into this gene circuit, it is expected that the pattern can be controlled by changes in temperature.