Difference between revisions of "Part:BBa K3259004"
 
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In this circuit, LasI and RhlI which works as activator and inhibitor respectively are both controlled by pLas-OR.
 
In this circuit, LasI and RhlI which works as activator and inhibitor respectively are both controlled by pLas-OR.
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PAI2 produced by LasI gene promotes the expression of both LasI and RhlI by binding to LasR. This makes PAI2 to be function as an activator for this Turing circuit.
+
PAI1 produced by LasI gene promotes the expression of both LasI and RhlI by binding to LasR. This makes PAI1 to be function as an activator for this Turing circuit.
−
Conversely, RhlI produces PAI1 and binds to RhlR to promote the expression of CI. This results in suppression of RhlI and LasI expression, therefore PAI1 works as an inhibitor in this Turing pattern circuit.
+
Conversely, RhlI produces PAI2 and binds to RhlR to promote the expression of CI. This results in suppression of RhlI and LasI expression, therefore PAI2 works as an inhibitor in this Turing pattern circuit.
−
Turing pattern is generated by this gene circuit by the interaction between these two substances, namely PAI2 and PAI1.
+
Turing pattern is generated by this gene circuit by the interaction between these two substances, namely PAI1 and PAI2.
  
 
Furthermore, since BBa_K3259000 part is also included into this gene circuit, it is expected that the pattern can be controlled by blue light.
 
Furthermore, since BBa_K3259000 part is also included into this gene circuit, it is expected that the pattern can be controlled by blue light.

Latest revision as of 02:17, 22 October 2019


composite part for Turing Pattern controlled by Blue light

In this circuit, LasI and RhlI which works as activator and inhibitor respectively are both controlled by pLas-OR. PAI1 produced by LasI gene promotes the expression of both LasI and RhlI by binding to LasR. This makes PAI1 to be function as an activator for this Turing circuit. Conversely, RhlI produces PAI2 and binds to RhlR to promote the expression of CI. This results in suppression of RhlI and LasI expression, therefore PAI2 works as an inhibitor in this Turing pattern circuit. Turing pattern is generated by this gene circuit by the interaction between these two substances, namely PAI1 and PAI2.

Furthermore, since BBa_K3259000 part is also included into this gene circuit, it is expected that the pattern can be controlled by blue light.

If you wish to know more about how Turing patterns are created, please read our Wiki (https://2019.igem.org/Team:TokyoTech/Experiments) Or read the paper in (1).


(1)David Karig, K. Michael Martini, Ting Lu, Nicholas A. DeLateur, Nigel Goldenfeld, and Ron Weiss (2018). Stochastic Turing patterns in a synthetic bacterial population . Proceedings of the National Academy of Sciences ,115(26), 6572-6577

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 58
    Illegal NheI site found at 1120
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1917
    Illegal BamHI site found at 3676
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 516
    Illegal AgeI site found at 713
    Illegal AgeI site found at 1479
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4151
    Illegal BsaI.rc site found at 5899