Difference between revisions of "Part:BBa K3002231"

 
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<partinfo>BBa_K3002231 short</partinfo>
 
<partinfo>BBa_K3002231 short</partinfo>
  
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This composite part contains a hygromycin resistance (BBa_K3002119) and the WT-PETase (BBa_K3002126) fused with an SP20His-tag for easy purification via Ni-NTA column and enhanced secretion.
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<html>
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This composite part contains a hygromycin resistance (<a href="https://parts.igem.org/Part:BBa_K3002119">BBa_K3002119</a>) and the WT-PETase (<a href="https://parts.igem.org/Part:BBa_K3002126">BBa_K3002126</a>) fused with an SP20His-tag for easy purification via Ni-NTA column and enhanced secretion.
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</html>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 02:04, 22 October 2019


L2 hygromycin resistance + WT-PETase_His

This composite part contains a hygromycin resistance (BBa_K3002119) and the WT-PETase (BBa_K3002126) fused with an SP20His-tag for easy purification via Ni-NTA column and enhanced secretion.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2801
    Illegal PstI site found at 3774
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2048
    Illegal PstI site found at 2801
    Illegal PstI site found at 3774
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1408
    Illegal XhoI site found at 250
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2801
    Illegal PstI site found at 3774
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2801
    Illegal PstI site found at 3774
    Illegal NgoMIV site found at 773
    Illegal NgoMIV site found at 955
    Illegal NgoMIV site found at 1326
    Illegal NgoMIV site found at 2536
    Illegal NgoMIV site found at 2563
    Illegal NgoMIV site found at 4133
  • 1000
    COMPATIBLE WITH RFC[1000]