Difference between revisions of "Part:BBa K3002229"
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<partinfo>BBa_K3002229 short</partinfo> | <partinfo>BBa_K3002229 short</partinfo> | ||
| − | This composite part contains a hygromycin resistance (BBa_K3002119) and the WT-PETase with the secretion signal cCA (BBa_K3002124) fused with an SP20His-tag for easy purification via Ni-NTA column and enhanced secretion. | + | <html> |
| + | This composite part contains a hygromycin resistance (<a href="https://parts.igem.org/Part:BBa_K3002119">BBa_K3002119</a>) and the WT-PETase with the secretion signal cCA (<a href="https://parts.igem.org/Part:BBa_K3002124">BBa_K3002124</a>) fused with an SP20His-tag for easy purification via Ni-NTA column and enhanced secretion. | ||
| + | </html> | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 02:03, 22 October 2019
L2 hygromycin resistance + cCA_WT-PETase_SP20His
This composite part contains a hygromycin resistance (BBa_K3002119) and the WT-PETase with the secretion signal cCA (BBa_K3002124) fused with an SP20His-tag for easy purification via Ni-NTA column and enhanced secretion.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 2868
Illegal PstI site found at 3841 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2048
Illegal PstI site found at 2868
Illegal PstI site found at 3841 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1408
Illegal XhoI site found at 250 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 2868
Illegal PstI site found at 3841 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 2868
Illegal PstI site found at 3841
Illegal NgoMIV site found at 773
Illegal NgoMIV site found at 955
Illegal NgoMIV site found at 1326
Illegal NgoMIV site found at 2603
Illegal NgoMIV site found at 2630
Illegal NgoMIV site found at 4332 - 1000COMPATIBLE WITH RFC[1000]