Difference between revisions of "Part:BBa K3002226"

 
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<partinfo>BBa_K3002226 short</partinfo>
 
<partinfo>BBa_K3002226 short</partinfo>
  
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This composite part contains a spectinomycin resistance (BBa_K3002102), the mutant PETase with the secretion signal ARS (BBa_K3002121) and a SP20His-tag and the wildtype MHETase with the secretion signal cCA (BBa_K3002114) fused with an SP20HA-tag for easy detection via antibody and enhanced secretion.
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This composite part contains a spectinomycin resistance (<a href="https://parts.igem.org/Part:BBa_K3002102">BBa_K3002102</a>), the mutant PETase with the secretion signal ARS (<a href="https://parts.igem.org/Part:BBa_K3002121">BBa_K3002121</a>) and a SP20His-tag and the wildtype MHETase with the secretion signal cCA (<a href="https://parts.igem.org/Part:BBa_K3002114">BBa_K3002114</a>) fused with an SP20HA-tag for easy detection via antibody and enhanced secretion.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 01:57, 22 October 2019


L2 spectinomycin resistance + ARS_Mut-PETase_SP20His + ccA_MHETase_ SP20HA

This composite part contains a spectinomycin resistance (BBa_K3002102), the mutant PETase with the secretion signal ARS (BBa_K3002121) and a SP20His-tag and the wildtype MHETase with the secretion signal cCA (BBa_K3002114) fused with an SP20HA-tag for easy detection via antibody and enhanced secretion.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5354
    Illegal PstI site found at 3491
    Illegal PstI site found at 4464
    Illegal PstI site found at 6696
    Illegal PstI site found at 7020
    Illegal PstI site found at 7363
    Illegal PstI site found at 8173
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5354
    Illegal NheI site found at 2665
    Illegal NheI site found at 5618
    Illegal PstI site found at 3491
    Illegal PstI site found at 4464
    Illegal PstI site found at 6696
    Illegal PstI site found at 7020
    Illegal PstI site found at 7363
    Illegal PstI site found at 8173
    Illegal NotI site found at 7031
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5354
    Illegal BglII site found at 7941
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5354
    Illegal PstI site found at 3491
    Illegal PstI site found at 4464
    Illegal PstI site found at 6696
    Illegal PstI site found at 7020
    Illegal PstI site found at 7363
    Illegal PstI site found at 8173
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5354
    Illegal PstI site found at 3491
    Illegal PstI site found at 4464
    Illegal PstI site found at 6696
    Illegal PstI site found at 7020
    Illegal PstI site found at 7363
    Illegal PstI site found at 8173
    Illegal NgoMIV site found at 1401
    Illegal NgoMIV site found at 1584
    Illegal NgoMIV site found at 1694
    Illegal NgoMIV site found at 3226
    Illegal NgoMIV site found at 3253
    Illegal NgoMIV site found at 4955
    Illegal NgoMIV site found at 6629
  • 1000
    COMPATIBLE WITH RFC[1000]