Difference between revisions of "Part:BBa K3002225"

 
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<partinfo>BBa_K3002225 short</partinfo>
 
<partinfo>BBa_K3002225 short</partinfo>
  
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This composite part contains a spectinomycin resistance (BBa_K3002102), the mutant PETase with the secretion signal GLE (BBa_K3002122) and a SP20His-tag and the wildtype MHETase with the secretion signal cCA (BBa_K3002114) fused with an SP20HA-tag for easy detection via antibody and enhanced secretion.
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This composite part contains a spectinomycin resistance (<a href="https://parts.igem.org/Part:BBa_K3002102">BBa_K3002102</a>), the mutant PETase with the secretion signal GLE (<a href="https://parts.igem.org/Part:BBa_K3002122">BBa_K3002122</a>) and a SP20His-tag and the wildtype MHETase with the secretion signal cCA (<a href="https://parts.igem.org/Part:BBa_K3002114">BBa_K3002114</a>) fused with an SP20HA-tag for easy detection via antibody and enhanced secretion.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 01:56, 22 October 2019


L2 spectinomycin resistance + GLE_Mut-PETase_SP20His + ccA_MHETase_SP20HA

This composite part contains a spectinomycin resistance (BBa_K3002102), the mutant PETase with the secretion signal GLE (BBa_K3002122) and a SP20His-tag and the wildtype MHETase with the secretion signal cCA (BBa_K3002114) fused with an SP20HA-tag for easy detection via antibody and enhanced secretion.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5366
    Illegal PstI site found at 3503
    Illegal PstI site found at 4476
    Illegal PstI site found at 6708
    Illegal PstI site found at 7032
    Illegal PstI site found at 7375
    Illegal PstI site found at 8185
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5366
    Illegal NheI site found at 2665
    Illegal NheI site found at 5630
    Illegal PstI site found at 3503
    Illegal PstI site found at 4476
    Illegal PstI site found at 6708
    Illegal PstI site found at 7032
    Illegal PstI site found at 7375
    Illegal PstI site found at 8185
    Illegal NotI site found at 7043
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5366
    Illegal BglII site found at 7953
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5366
    Illegal PstI site found at 3503
    Illegal PstI site found at 4476
    Illegal PstI site found at 6708
    Illegal PstI site found at 7032
    Illegal PstI site found at 7375
    Illegal PstI site found at 8185
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5366
    Illegal PstI site found at 3503
    Illegal PstI site found at 4476
    Illegal PstI site found at 6708
    Illegal PstI site found at 7032
    Illegal PstI site found at 7375
    Illegal PstI site found at 8185
    Illegal NgoMIV site found at 1401
    Illegal NgoMIV site found at 1584
    Illegal NgoMIV site found at 1694
    Illegal NgoMIV site found at 3238
    Illegal NgoMIV site found at 3265
    Illegal NgoMIV site found at 4967
    Illegal NgoMIV site found at 6641
  • 1000
    COMPATIBLE WITH RFC[1000]