Difference between revisions of "Part:BBa K3042000"
| Line 12: | Line 12: | ||
mRFP1- monomeric derivative of Dsred (Campbell et al., 2002) | mRFP1- monomeric derivative of Dsred (Campbell et al., 2002) | ||
| − | <p>Brittany N. Sprecher, Huan Zhang, Senjie Lin (2019, April 9). Nuclear gene transformation in a dinoflagellate. doi: 10.1101/602821<p> | + | <p>Brittany N. Sprecher, Huan Zhang, Senjie Lin (2019, April 9). Nuclear gene transformation in a dinoflagellate. doi: 10.1101/602821</p> |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 01:48, 22 October 2019
Dinoflagellate Codon Optimized RFP
This part is a codon optimized version of RFP for use in use in Dinoflagellates, a single celled organism. This part was created to be used in DinoIII, a dinoflagellate backbone vector . The sequence for this part was synthesized via Snapgene. Considering dinoflagellates have preference when it comes to specific amino acids, the favorable codons of the dinoflagellates were chosen using the chart below to ensure the successful translation of the red fluorescent protein. This part can be used as a reporter/indicator of a successful transformation. Positive results are demonstrated by red fluorescence emitted from the cells.
Figure 1. Codon Optimized Chart for Oxyrrhis marina
This was the chart we used to design the RFP part in Snapgene, to be edited into the Dino III plasmid in place of the existing GFP part BBa_K3042005
mRFP1- monomeric derivative of Dsred (Campbell et al., 2002)
Brittany N. Sprecher, Huan Zhang, Senjie Lin (2019, April 9). Nuclear gene transformation in a dinoflagellate. doi: 10.1101/602821
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 13
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 696
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 13
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 13
- 1000COMPATIBLE WITH RFC[1000]