Difference between revisions of "Part:BBa K2669002:Experience"

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===Applications of BBa_K2669002 ===
 
===Applications of BBa_K2669002 ===
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The construct [[BBa_K3189015]] containing the chromoprotein amilCP ([[BBa_K2669002]]) under the control of [[BBa_K3189001]]. When the system is induced with 100 ng/mL of tetracycline, a dark blue colour is produced (Figure 4 and Figure 5). This shows [[BBa_K3189001]] is able to function with different reporter proteins other than just <i>gfp</i>.
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https://static.igem.org/mediawiki/parts/thumb/f/f5/T--Guelph--pTA-tetnotet.jpg/216px-T--Guelph--pTA-tetnotet.jpg
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<b>Figure 4: [[BBa_K2669002]] under the control of [[BBa_K3189001]].</b> amilCP expression being induced using 100 ng/mL tetracycline (left) and uninduced (right).
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https://static.igem.org/mediawiki/parts/thumb/2/29/T--Guelph--pTAtetnotetspun.jpg/207px-T--Guelph--pTAtetnotetspun.jpg
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<b>Figure 5: Pellets of cells of [[BBa_K2669002]] under the control of [[BBa_K3189001]] induced and uninduced with tetracycline.</b> Pellet of cells induced with 100 ng/mL tetracycline (left) and pellet of cells uninduced (right).
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===User Reviews===
 
===User Reviews===

Revision as of 01:45, 22 October 2019

Applications of BBa_K2669002


The construct BBa_K3189015 containing the chromoprotein amilCP (BBa_K2669002) under the control of BBa_K3189001. When the system is induced with 100 ng/mL of tetracycline, a dark blue colour is produced (Figure 4 and Figure 5). This shows BBa_K3189001 is able to function with different reporter proteins other than just gfp.

216px-T--Guelph--pTA-tetnotet.jpg
Figure 4: BBa_K2669002 under the control of BBa_K3189001. amilCP expression being induced using 100 ng/mL tetracycline (left) and uninduced (right).

207px-T--Guelph--pTAtetnotetspun.jpg
Figure 5: Pellets of cells of BBa_K2669002 under the control of BBa_K3189001 induced and uninduced with tetracycline. Pellet of cells induced with 100 ng/mL tetracycline (left) and pellet of cells uninduced (right).

User Reviews

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