Difference between revisions of "Part:BBa K3046009:Design"

 
 
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===Design Notes===
 
===Design Notes===
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The placement of the promoter in relation to the mCherry was chosen to allow for expression in both bacteria and eukaryotes. Furthermore, mCherry was chosen specifically to allow for protein production in both pro- and eukaryotes, which enables the device's screening function as described in the 'Usage & Biology' section on the main page.
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===Source===
 
===Source===
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This device was assembled from <i>de novo</i> syntethesized fragments using Golden Gate assembly with the SapI restriction enzyme.
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===References===
 
===References===

Latest revision as of 01:43, 22 October 2019


Fungal MoClo promoter test device


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1158
    Illegal BamHI site found at 1418
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 232
    Illegal BsaI.rc site found at 6


Design Notes

The placement of the promoter in relation to the mCherry was chosen to allow for expression in both bacteria and eukaryotes. Furthermore, mCherry was chosen specifically to allow for protein production in both pro- and eukaryotes, which enables the device's screening function as described in the 'Usage & Biology' section on the main page.

Source

This device was assembled from de novo syntethesized fragments using Golden Gate assembly with the SapI restriction enzyme.

References