Difference between revisions of "Part:BBa K3002211"

 
Line 3: Line 3:
 
<partinfo>BBa_K3002211 short</partinfo>
 
<partinfo>BBa_K3002211 short</partinfo>
  
−
This composite part contains a spectinomycin resistance (BBa_K3002102), the mutant PETase with the secretion signal GLE (BBa_K3002107) and the MHETase with the cCA (BBa_K3002113), both fused with an HA-tag for easy detection via HA-antibody.
+
<html>
 +
This composite part contains a spectinomycin resistance (<a href="https://parts.igem.org/Part:BBa_K3002102">BBa_K3002102</a>), the mutant PETase with the secretion signal GLE (<a href="https://parts.igem.org/Part:BBa_K3002107">BBa_K3002107</a>) and the MHETase with the cCA (<a href="https://parts.igem.org/Part:BBa_K3002113">BBa_K3002113</a>), both fused with an HA-tag for easy detection via HA-antibody.
 +
</html>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 01:36, 22 October 2019


L2 spectinomycin resistance + GLE_Mut-PETase + cCA_MHETase

This composite part contains a spectinomycin resistance (BBa_K3002102), the mutant PETase with the secretion signal GLE (BBa_K3002107) and the MHETase with the cCA (BBa_K3002113), both fused with an HA-tag for easy detection via HA-antibody.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5315
    Illegal PstI site found at 3503
    Illegal PstI site found at 4476
    Illegal PstI site found at 6657
    Illegal PstI site found at 6981
    Illegal PstI site found at 7324
    Illegal PstI site found at 8134
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5315
    Illegal NheI site found at 2665
    Illegal NheI site found at 5579
    Illegal PstI site found at 3503
    Illegal PstI site found at 4476
    Illegal PstI site found at 6657
    Illegal PstI site found at 6981
    Illegal PstI site found at 7324
    Illegal PstI site found at 8134
    Illegal NotI site found at 6992
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5315
    Illegal BglII site found at 7902
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5315
    Illegal PstI site found at 3503
    Illegal PstI site found at 4476
    Illegal PstI site found at 6657
    Illegal PstI site found at 6981
    Illegal PstI site found at 7324
    Illegal PstI site found at 8134
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5315
    Illegal PstI site found at 3503
    Illegal PstI site found at 4476
    Illegal PstI site found at 6657
    Illegal PstI site found at 6981
    Illegal PstI site found at 7324
    Illegal PstI site found at 8134
    Illegal NgoMIV site found at 1401
    Illegal NgoMIV site found at 1584
    Illegal NgoMIV site found at 1694
    Illegal NgoMIV site found at 3238
    Illegal NgoMIV site found at 3265
    Illegal NgoMIV site found at 4916
    Illegal NgoMIV site found at 6590
  • 1000
    COMPATIBLE WITH RFC[1000]