Difference between revisions of "Part:BBa K2992013"

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===Characterisation===
 
===Characterisation===
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This basic part was used in p<i>botR</i> to prevent run through expression from the pyrE operon through to the <i>botR</i> gene, therefore preventing any basal levels of BotR expression. See our [https://2019.igem.org/Team:Nottingham/Results results page] for more information.  
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This basic part was used upstream of P<i>botR</i> to prevent chromosomal read-through from the <i>pyrKDE<i> operon, upon integration of the P<i>botR</i>-<i>botR</i> module. See our [https://2019.igem.org/Team:Nottingham/Results results page] for more information.  
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https://2019.igem.org/wiki/images/5/5e/T--Nottingham--Basic4.png
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https://2019.igem.org/wiki/images/6/6b/T--Nottingham--Basic3.png
 
  
 
===References===
 
===References===

Revision as of 01:34, 22 October 2019

Usage

Tfad is a terminator sequence derived from the fad gene of C. tetani, encoding FAD-binding oxidreducatse. These enzymes catalyse the conversion of dihydroxyacetone phosphate to glycerol-3-phosphate in a reversible reaction. In our project we use this strong terminator to prevent polar transcription from the pyrKDE operon of C. sporogenes, following chromosomal knock-in of botR in part BBa_K2992025, at this locus. The secondary structure for Tfad was predicted using the Mfold web tool (Zuker, 2003).

Terminator.png

Tfad from C. tetani

  • Transcriptional terminator sequence from the FAD-dependent oxidoreductase, fad, from Clostridium tetani

Secondary Structure

Mfold-K2992013-1.png

Characterisation

This basic part was used upstream of PbotR to prevent chromosomal read-through from the pyrKDE<i> operon, upon integration of the P<i>botR-botR module. See our results page for more information.

References

Zuker, M. (2003). Mfold web server for nucleic acid folding and hybridization prediction. Nucleic Acids Research, 31(13), pp.3406-3415.


Measurement

  • [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]