Difference between revisions of "Part:BBa K3156888"

Revision as of 01:33, 22 October 2019

pLac Promoter-ssDNA[sfGFP(ON)]-Ec86-Beta

Design

We combined msd-msr cassette, Reverse Transcriptase Ec-86 and Beta recombinase together to create BBa_K3156888. Msd-msr cassette can produce mRNA for reverse transcription to produce

We created a

**Figure 2.Stimulated reparing progress of K3156888.**

Usage and Biology

Culturing and plating

For each experiment, the samples were separately inoculated in LB medium plus appropriate antibiotics and grown overnight at 37°C, 200 revolutions per minute (RPM) to obtain seed cultures. Unless otherwise noted,inductions were performed by diluting the seed cultures (1:1000) in 2 ml of prewarmed LB plus appropriate antibiotics with or without inducers followed by 24 hours incubation 30°C, 700 RPM.

**Figure 1.Repairing result of BBa_K3156888(*sfgfp* forward).**

**Figure 1.Repairing result of BBa_K3156888(*sfgfp* reverse).**

Reference

[1]F. Farzadfard, T. K. Lu, Science 346,1256272 (2014). DOI: 10.1126/science.1256272

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 9
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 1
Illegal XhoI site found at 516
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 ===Usage and Biology===
 <html>
+<h3 id="CBD">Culturing and plating</h3>
+<p>For each experiment, the samples were separately inoculated in LB medium plus appropriate antibiotics and grown overnight at 37°C, 200 revolutions per minute (RPM) to obtain seed cultures. Unless otherwise noted,inductions were performed by diluting the seed cultures (1:1000) in 2 ml of prewarmed LB plus appropriate antibiotics with or without inducers followed by 24 hours incubation 30°C, 700 RPM.
 <figure>
 <p style="text-align:center;"><img src="https://2019.igem.org/wiki/images/0/06/T--SHSBNU_China---dual_repair.png" width = "700" height ="500"/>