Difference between revisions of "Part:BBa K3201023"

Latest revision as of 01:32, 22 October 2019

STAR target RFP

This is an RFP coding device with a STAR Target inserted upstream. In this way, the RFP is only transcribed in the presence of a STAR molecule.

Usage and Biology

We transformed bacteria with this construct to observe the function of the device in vivo. In the absence of a STAR, most colonies appear white, as shown in Figure 1.

Figure 1: Bacteria with STARtarget RFP (no STAR) construct

However, when a construct carrying a STAR is added, then more red colonies appear, as shown in Figure 2.

Figure 2: Original STAR colonies.

We conclude that the construct works as expected. Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 702
Illegal AgeI site found at 814
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 This is an RFP coding device with a STAR Target inserted upstream. In this way, the RFP is only transcribed in the presence of a STAR molecule.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
-<!-- -->
+We transformed bacteria with this construct to observe the function of the device in vivo. In the absence of a STAR, most colonies appear white, as shown in Figure 1.
+[[File:T--Athens--STARsolo.png|400px|thumb|center|Figure 1: Bacteria with STARtarget RFP (no STAR) construct]]
+However, when a construct carrying a STAR is added, then more red colonies appear, as shown in Figure 2.
+[[File:T--Athens--STARoriginal.png|400px|thumb|center|Figure 2: Original STAR colonies.]]
+We conclude that the construct works as expected.
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K3201023 SequenceAndFeatures</partinfo>