Difference between revisions of "Part:BBa K3202022"

Revision as of 01:31, 22 October 2019

msfGFP

We introduced this new part to the iGEM parts, and have characterized this part in several of our composite parts. This part is used to demonstrate the function of our bistable system.

Characterization

The newly introduced msfGFP is very sensitive according to our results. Comparing to BN006/Contains BBa_K3202029, this new part shows significant difference in both AraC-pBAD inducer system and Xyls-Pm inducer system. Qualitative and quantitative results are shown below.

Figure 1. The left centrifuge tube contains BN006(backbone), and the one on the right contains BN072(AraC-pBAD-msfGFP)

Figure 2. The left centrifuge tube contains BN006(backbone), and the one on the right contains BN073(Xyls-Pm-msfGFP)

Figure 1. The left centrifuge tube contains BN006(backbone), and the one on the right contains BN072(AraC-pBAD-msfGFP)

Figure 2. The left centrifuge tube contains BN006(backbone), and the one on the right contains BN073(Xyls-Pm-msfGFP)

</br> </br> </br> </br> </br> </br> </br> </br> Also, comparing to sfGFP, this new part shows significant difference in both AraC-pBAD inducer system and Xyls-Pm inducer system as well. Quantitative results are shown below.

Figure 3. BN007(AraC-pBAD-sfGFP) and BN072(AraC-pBAD-msfGFP), noticeable difference is shown.

Figure 4. BN009(Xyls-Pm-sfGFP) and BN073(Xyls-Pm-msfGFP), noticeable difference is shown.

Sequence and Features