Difference between revisions of "Part:BBa K3002207"
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<partinfo>BBa_K3002207 short</partinfo> | <partinfo>BBa_K3002207 short</partinfo> | ||
− | This composite part contains a spectinomycin resistance (BBa_K3002102) | + | <html> |
+ | This composite part contains a spectinomycin resistance (<a href="https://parts.igem.org/Part:BBa_K3002102">BBa_K3002102</a>)and the Mutant PETase (<a href="https://parts.igem.org/Part:BBa_K3002107">BBa_K3002107</a>) fused with an HA-tag for easy detection via HA-antibody and with the secretion signal GLE. | ||
+ | </html> | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 01:29, 22 October 2019
L2 spectinomycin resistance + GLE_Mut-PETase
This composite part contains a spectinomycin resistance (BBa_K3002102)and the Mutant PETase (BBa_K3002107) fused with an HA-tag for easy detection via HA-antibody and with the secretion signal GLE.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 2401
Illegal PstI site found at 3503
Illegal PstI site found at 4476 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 2401
Illegal NheI site found at 2665
Illegal PstI site found at 3503
Illegal PstI site found at 4476 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 2401
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 2401
Illegal PstI site found at 3503
Illegal PstI site found at 4476 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 2401
Illegal PstI site found at 3503
Illegal PstI site found at 4476
Illegal NgoMIV site found at 1401
Illegal NgoMIV site found at 1584
Illegal NgoMIV site found at 1694
Illegal NgoMIV site found at 3238
Illegal NgoMIV site found at 3265
Illegal NgoMIV site found at 4916 - 1000COMPATIBLE WITH RFC[1000]