Difference between revisions of "Part:BBa K3192000:Design"

 
 
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===Design Notes===
 
===Design Notes===
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Designed using DeVovo DNA software and synthesized.  
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<p>Denovo DNA was used for design of the Ribosomal Binding site. Given the coding sequence for the specified gene, DeNovo ran thousands of iterations to develop a RBS with high translation initiation rates. This sequence was then synthesized (for the 2019 Virginia iGEM team it was synthesized with its respective coding region). Sequencing confirmed the identity of the synthesized construct. </p>
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===Source===
 
===Source===
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===References===
 
===References===
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https://www.denovodna.com:4433

Latest revision as of 01:26, 22 October 2019


Custom RBS Optimized for BBa_K3192020


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Denovo DNA was used for design of the Ribosomal Binding site. Given the coding sequence for the specified gene, DeNovo ran thousands of iterations to develop a RBS with high translation initiation rates. This sequence was then synthesized (for the 2019 Virginia iGEM team it was synthesized with its respective coding region). Sequencing confirmed the identity of the synthesized construct.


Source

Synthetic Source

References

https://www.denovodna.com:4433