Difference between revisions of "Part:BBa K3002200"

 
Line 3: Line 3:
 
<partinfo>BBa_K3002200 short</partinfo>
 
<partinfo>BBa_K3002200 short</partinfo>
  
−
This composite part contains a spectinomycin resistance (BBa_K3002102), the mutant PETase (BBa_K3002105) and the wildtype MHETase (BBa_K3002112), both are fused with an HA-tag for easy detection via HA-antibody.
+
<html>
 +
This composite part contains a spectinomycin resistance (<a href="https://parts.igem.org/Part:BBa_K3002102">BBa_K3002102</a>), the mutant PETase (<a href="https://parts.igem.org/Part:BBa_K3002105">BBa_K3002105</a>) and the wildtype MHETase (<a href="https://parts.igem.org/Part:BBa_K3002112">BBa_K3002112</a>), both are fused with an HA-tag for easy detection via HA-antibody.
 +
</html>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 01:24, 22 October 2019


L2 spectinomycin resistance + Mut-PETase + MHETase

This composite part contains a spectinomycin resistance (BBa_K3002102), the mutant PETase (BBa_K3002105) and the wildtype MHETase (BBa_K3002112), both are fused with an HA-tag for easy detection via HA-antibody.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5370
    Illegal PstI site found at 3558
    Illegal PstI site found at 4531
    Illegal PstI site found at 6785
    Illegal PstI site found at 7109
    Illegal PstI site found at 7452
    Illegal PstI site found at 8262
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5370
    Illegal NheI site found at 2680
    Illegal NheI site found at 5649
    Illegal PstI site found at 3558
    Illegal PstI site found at 4531
    Illegal PstI site found at 6785
    Illegal PstI site found at 7109
    Illegal PstI site found at 7452
    Illegal PstI site found at 8262
    Illegal NotI site found at 7120
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5370
    Illegal BglII site found at 8030
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5370
    Illegal PstI site found at 3558
    Illegal PstI site found at 4531
    Illegal PstI site found at 6785
    Illegal PstI site found at 7109
    Illegal PstI site found at 7452
    Illegal PstI site found at 8262
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5370
    Illegal PstI site found at 3558
    Illegal PstI site found at 4531
    Illegal PstI site found at 6785
    Illegal PstI site found at 7109
    Illegal PstI site found at 7452
    Illegal PstI site found at 8262
    Illegal NgoMIV site found at 1401
    Illegal NgoMIV site found at 1584
    Illegal NgoMIV site found at 1694
    Illegal NgoMIV site found at 3293
    Illegal NgoMIV site found at 3320
    Illegal NgoMIV site found at 4971
    Illegal NgoMIV site found at 6718
  • 1000
    COMPATIBLE WITH RFC[1000]