Difference between revisions of "Part:BBa K3156888"

Revision as of 01:23, 22 October 2019

pLac Promoter-ssDNA[sfGFP(ON)]-Ec86-Beta

Design

We combined msd-msr cassette, Reverse Transcriptase Ec-86 and Beta recombinase together to create BBa_K3156888. Msd-msr cassette can produce mRNA for reverse transcription to produce

We created a

**Figure 2.Stimulated reparing progress of K3156888.**

Usage and Biology

**Figure 1.Repairing result of BBa_K3156888(*sfgfp* forward).**

**Figure 1.Repairing result of BBa_K3156888(*sfgfp* reverse).**

Reference

[1]F. Farzadfard, T. K. Lu, Science 346,1256272 (2014). DOI: 10.1126/science.1256272

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 9
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 1
Illegal XhoI site found at 516
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 28: / Line 28: @@
 <figcaption></p>
 <p style="text-align:center;"><b>Figure 1.Repairing result of BBa_K3156888(<i>sfgfp</i> forward).</b> </p>
+</figcaption>
+</figure>
+<figure>
+<p style="text-align:center;"><img src="https://2019.igem.org/wiki/images/b/b0/T--SHSBNU_China---ssdna-result.png" width = "800" height ="400"/>
+<figcaption></p>
+<p style="text-align:center;"><b>Figure 1.Repairing result of BBa_K3156888(<i>sfgfp</i> reverse).</b> </p>
 </figcaption>
 </figure>
 <b>Reference</b>
 <p>[1]F. Farzadfard, T. K. Lu, Science 346,1256272 (2014). DOI: 10.1126/science.1256272</p>