Difference between revisions of "Part:BBa K3139012:Design"

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===References===
 
===References===
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[1]Jianguo Y , Xiaqing X , Nan X , et al. Polyprotein strategy for stoichiometric assembly of nitrogen fixation components for synthetic biology[J]. Proceedings of the National Academy of Sciences, 2018:201804992-.
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[1]Cesaratto F , Burrone O R , Petris G . Tobacco Etch Virus protease: A shortcut across biotechnologies[J]. Journal of Biotechnology, 2016, 231.<br>
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[2]Jianguo Y , Xiaqing X , Nan X , et al. Polyprotein strategy for stoichiometric assembly of nitrogen fixation components for synthetic biology[J]. Proceedings of the National Academy of Sciences, 2018:201804992-.

Latest revision as of 01:23, 22 October 2019


TEVpS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 322
    Illegal SapI.rc site found at 670


Design Notes

We need to control the ratio of enzyme to fusion protein expression,to make the best effect.


Source

We found the coding sequence of TEVpS on NCBI,then send it for syhthesis.

References

[1]Cesaratto F , Burrone O R , Petris G . Tobacco Etch Virus protease: A shortcut across biotechnologies[J]. Journal of Biotechnology, 2016, 231.
[2]Jianguo Y , Xiaqing X , Nan X , et al. Polyprotein strategy for stoichiometric assembly of nitrogen fixation components for synthetic biology[J]. Proceedings of the National Academy of Sciences, 2018:201804992-.