Difference between revisions of "Part:BBa K3292001"
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This device uses Bba_K2406020, Bba_J34801 and Bba_0010 to regulate the expression of a super folding GFP (Bba_I746916). The main objective of creating this device is to facilitate the secretion and characterization of any protein intracellularly produced after adding its nucleotide sequence next to the sfGFP.
 
This device uses Bba_K2406020, Bba_J34801 and Bba_0010 to regulate the expression of a super folding GFP (Bba_I746916). The main objective of creating this device is to facilitate the secretion and characterization of any protein intracellularly produced after adding its nucleotide sequence next to the sfGFP.
  
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The characterization of this part included a Bradford assay to quantify the amount of protein present in the medium for each of the proposed constructs. The conditions for the assay are listed below.
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The other characterization assay was a fluorescent measurement, bacteria were growth at 30ºC for 3 hrs. Then
  
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<! -- Usage and Biology
 
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The characterization of this part included a Bradford assay to quantify the amount of protein present in the medium for each of the proposed constructs.
 
  
  

Revision as of 01:22, 22 October 2019


T7-LacO Promoter + strong RBS + sialidase + sfGFP + 6x his-tag + double terminator


This device uses Bba_K2406020, Bba_J34801 and Bba_0010 to regulate the expression of a super folding GFP (Bba_I746916). The main objective of creating this device is to facilitate the secretion and characterization of any protein intracellularly produced after adding its nucleotide sequence next to the sfGFP.


The characterization of this part included a Bradford assay to quantify the amount of protein present in the medium for each of the proposed constructs. The conditions for the assay are listed below.

The other characterization assay was a fluorescent measurement, bacteria were growth at 30ºC for 3 hrs. Then


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 721
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1986
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 646
    Illegal BsaI.rc site found at 510
    Illegal BsaI.rc site found at 1647
    Illegal SapI.rc site found at 2055