Difference between revisions of "Part:BBa K117004:Experience"

(Characterization of pLacI-GFP)
 
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=='''Abnormality found in the registry'''==
 
=='''Abnormality found in the registry'''==
 
This device could be found in the registry in the form of [https://parts.igem.org/wiki/index.php?title=Part:BBa_J04430 BBa_J04430]. However, the team found an abnormality when BBa_J04430 was transformed into Top 10 cells. As shown on the pictures below, the cells glow pink instead of green under UV light.
 
This device could be found in the registry in the form of [https://parts.igem.org/wiki/index.php?title=Part:BBa_J04430 BBa_J04430]. However, the team found an abnormality when BBa_J04430 was transformed into Top 10 cells. As shown on the pictures below, the cells glow pink instead of green under UV light.

Latest revision as of 01:01, 27 October 2008

Abnormality found in the registry

This device could be found in the registry in the form of BBa_J04430. However, the team found an abnormality when BBa_J04430 was transformed into Top 10 cells. As shown on the pictures below, the cells glow pink instead of green under UV light.

BBa_J04430 glows pink


Characterization of pLacI-GFP

The NTU@iGEM team characterized the biobrick part pLacI-GFP. The influence of 1)concentration of Lactose 2) temperature and 3) time were investigated.

The characterization overlook

Shown below is the graph obtained for the experiment conducted at 37°C.

Characterization at 37°C

Electron microscope pictures

Cells induced with 1mM lactose concentration
Cells induced with 10mM lactose concentration


For more information including the protocols and results obtained, please go to: [http://2008.igem.org/Team:NTU-Singapore/Parts/Characterization_of_LacI-GFP NTU@iGEM Characterization of pLac-GFP]

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